Caracterización de dos comunidades microbianas de ambientes extremos ácidos del Parque Nacional Volcán Rincón de la Vieja, Guanacaste, Costa Rica
| dc.contributor.advisor | Mora López, Marielos | |
| dc.contributor.author | Hernández Ascencio, Walter Ismael | es_CR |
| dc.date.accessioned | 2015-10-05T17:11:33Z | |
| dc.date.accessioned | 2021-09-10T00:12:14Z | |
| dc.date.available | 2015-10-05T17:11:33Z | |
| dc.date.available | 2021-09-10T00:12:14Z | |
| dc.date.issued | 2012 | es_CR |
| dc.description | Tesis (maestría académica en microbiología)--Universidad de Costa Rica. Sistema de Estudios de Posgrado, 2012 | es_CR |
| dc.description.abstract | First description of a microbial community inhabiting an extreme acidic environment at Las Pailas geothermal field, Rincon de la Vieja Volcano National Park, Costa Rica. Extremophilic microorganisms are those that thrive in environments with extremes of temperature, salinity, pH or concentration of heavy metals, among others. They are interesting for their unique ecology and physiology and their potential biotechnological applications. Tropical regions are hot spots of extremophile microbial diversity, however, they are highly unreported in the scientific literature. In Costa Rica, for example, there are only a few reports about studies conducted in acidic environments associated with Poas and Rincon de la Vieja volcanoes. This paper describes the composition of a microbial community inhabiting the edge of an acidic hot mud pool that belongs to a set of acidic hydrothermal manifestations, known as Las Pailas geothermal field, located in the Rincon de la Vieja Volcano National Park. The aim is to increase the knowledge about diversity of Costa Rican and tropical extremophilic microorganisms. The microbial community was evident as a green film on the surface of a mud accumulation with a pH of 2.5, high concentrations of sulfate and metal ions and exposed to hot vapors from the mud pool. The microorganisms were observed by light microscopy, transmission electron microscopy and scanning electron microscopy. 16S rDNA librarles were constructed for chloroplast, Bacteria and Archaea. They were grouped according to restriction fragment length polymorphisms (RFLPs) anda representative of each cluster was sequenced. Sequences were aligned to built phylogenetic trees that showed severa] of the microbia] groups present in the sample. Microscopic observations revealed that the community was dominated by Cyanidiales algae and that pennate diatoms and prokaryotes also were present. 16S rDNA sequences were associaed mostly with enviromental clones from sites... | es_CR |
| dc.description.procedence | UCR::Investigación::Sistema de Estudios de Posgrado::Salud::Maestría Académica en Microbiología | es_CR |
| dc.identifier.uri | https://repositorio.sibdi.ucr.ac.cr/handle/123456789/2041 | |
| dc.language.iso | spa | es_CR |
| dc.subject | MICROORGANISMOS - INVESTIGACIONES | es_CR |
| dc.subject | DIVERSIDAD BACTERIANA - INVESTIGACIONES - PARQUE NACIONAL VOLCAN RINCON DE LA VIEJA , COSTA RICA | es_CR |
| dc.subject | DIVERSIDAD MICROBIANA - INVESTIGACIONES - PARQUE NACIONAL VOLCAN RINCON DE LA VIEJA, COSTA RICA | es_CR |
| dc.subject | DIVERSIDAD MICROBIANA - INVESTIGACIONES - LAS PAILAS (GUANACASTE, COSTA RICA) | es_CR |
| dc.subject | ACIDOS | es_CR |
| dc.title | Caracterización de dos comunidades microbianas de ambientes extremos ácidos del Parque Nacional Volcán Rincón de la Vieja, Guanacaste, Costa Rica | es_CR |
| dc.type | tesis de maestría | es_CR |
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